Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Representative patterns of DAXX, DRD3, and DISC1 expression in chondrosarcoma (CS) and osteochondroma (OC) tissues are shown (×200 original magnification).

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: DAXX, DRD3, and DISC1 expression in chondrosarcoma and osteochondroma.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Associations of DAXX, DRD3, and DISC1 expression with the clinicopathological characteristics of chondrosarcoma.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Associations of DAXX, DRD3, and DISC1 expression with survival in patients with chondrosarcoma. Prognoses are depicted with Kaplan–Meier curves. (A) DAXX expression (mean survival, positive 30.41 vs. negative 42.13 months; P = 0.012). (B) DRD3 expression (mean survival, positive 30.87 vs. negative 40.85 months; P = 0.018). (C) DISC1 expression (mean survival, positive 34.09 vs. negative 37.34 months; P = 0.404).

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Relationships of DAXX, DRD3, and DISC1 expression and clinicopathological characteristics to average survival in patients with chondrosarcoma.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Associations of DAXX, DRD3, and DISC1 expression and clinicopathological characteristics with overall survival in patients with chondrosarcoma, as determined by multivariable Cox regression.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Competing risks regression models for 80 patients with CS.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques:

Journal: Frontiers in Molecular Biosciences

Article Title: Association of Mental Health-Related Proteins DAXX, DRD3, and DISC1 With the Progression and Prognosis of Chondrosarcoma

doi: 10.3389/fmolb.2019.00134

Figure Lengend Snippet: Receiver operating characteristic curves for DAXX, DRD3, and DISC1 protein expression in 80 patients with chondrosarcoma (January 2011–June 2015). Areas under the curves are 0.673, 0.670, and 0.688, respectively.

Article Snippet: The tissue sections were then incubated with rabbit anti-human DAXX, DRD3, and DISC1 antibodies (Boster Biological Technology Co., Ltd., California, USA) for 2 h, followed by rinsing with PBS three times for 5 min each.

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Naca and Lrp6 form part of a common genetic pathway regulating bone mass. Femurs of female mice were analyzed by μCT at 3 months of age. BV/TV, bone volume/tissue volume. * p < 0.05, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: The osteoanabolic response to iPTH is inhibited in Naca / Lrp6 compound heterozygotes. Four-month-old female mice were treated once daily, 5 days/week for 1 month with 100 μg/kg of PTH(1-34). Vertebrae were analyzed by μCT. BV/TV, bone volume/tissue volume. Results are expressed as change from vehicle-treated animals, which are ascribed a value of 100%. * p < 0.05, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: The increase in biomechanical properties induced by iPTH treatment is blunted in Naca / Lrp6 compound heterozygotes. Four-month-old female mice were treated once daily, 5 days/week for 1 month with 100 μg/kg of PTH(1-34). L2 vertebrae were analyzed by compression testing to measure stiffness ( A ), load at yield ( B ) and maximum load ( C ). Results are expressed as change from vehicle-treated animals, which are ascribed a value of 100%. * p < 0.05; ** p < 0.01; # p < 0.0001, two-way analysis of variance (ANOVA) with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Gene expression monitoring in control ( Naca 99S/S ; Lrp6 +/+ ;OCN-Cre) and compound heterozygous ( Naca 99S/A ; Lrp6 +/fl ;OCN-Cre) littermates. RNA was extracted from marrow-flushed tibial diaphyses, reverse-transcribed and analyzed by RT-qPCR. Relative expression of Lrp6 ( A ), Alpl ( B ) and Col1a1 ( C ) is shown. * p < 0.05; ** p < 0.01; *** p < 0.001, two-way ANOVA with post hoc tests.

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques: Gene Expression, Control, Reverse Transcription, Quantitative RT-PCR, Expressing

Journal: International Journal of Molecular Sciences

Article Title: NACA and LRP6 Are Part of a Common Genetic Pathway Necessary for Full Anabolic Response to Intermittent PTH

doi: 10.3390/ijms23020940

Figure Lengend Snippet: Parallel PTH signaling pathways in control ( Naca 99S/S ; Lrp6 +/+ ;OCN-Cre) and compound heterozygous ( Naca 99S/A ; Lrp6 +/fl ;OCN-Cre) littermates. Protein extracts were prepared from tibial shafts and immunoprobed for β-Catenin ( A ) and SIK2 ( B ). Equivalent loading was assessed by probing for α-Tubulin ( C ).

Article Snippet: Real-time qPCR using TaqMan universal PCR master mix and gene-specific Taqman assays for Lrp6 (Mm00521783_m1), alkaline phosphatase ( Alpl , Mm00475834_m1), and the alpha 1 chain of type I collagen ( Col1a1 , Mm00801666_g1) was performed using a QuantStudio 7 real-time PCR system (Life Technologies Applied Biosystems).

Techniques: Protein-Protein interactions, Control